Metadata of CSIR Papers
View Archive Info| Field | Value | |
| Creator |
Basundra, R
Kumar, A Amrane, S Verma, A Phan, AT Chowdhury, S |
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| Subject |
Biochemistry & Molecular Biology
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| Description |
G-quadruplex motifs constitute unusual DNA secondary structures formed by stacking of planar hydrogen-bonded G-tetrads. Recent genome-wide bioinformatics and experimental analyses have suggested the interesting possibility that G-quadruplex motifs could be cis-regulatory elements. Here, we identified a characteristic potential G-quadruplex-forming sequence element within the promoter of human thymidine kinase 1 (TK1). Our NMR, UV and CD spectroscopy and gel electrophoresis data suggested that this sequence forms a novel intramolecular G-quadruplex with two G-tetrads in K+ solution. The results presented here indicate the role of this G-quadruplex motif in transcription of TK1 in cell-based reporter assays. Specific nucleotide substitutions designed to destabilize the G-quadruplex motif resulted in increased promoter activity, supporting direct involvement of the G-quadruplex motif in transcription of TK1. These studies suggest that the G-quadruplex motif may be an important target for controlling critical biological processes, such as DNA synthesis, mediated by TK1.
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| Publisher |
WILEY-BLACKWELL PUBLISHING, INCMALDENCOMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA
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| Date |
2011-09-20T12:07:05Z
2011-09-20T12:07:05Z 2010 |
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| Type |
Article
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| Identifier |
FEBS JOURNAL
1742-464X http://hdl.handle.net/123456789/13095 |
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| Language |
English
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