Metadata of CSIR Papers
View Archive Info| Field | Value | |
| Creator |
Sethi, D
Kumar, A Gandhi, RP Kumar, P Gupta, KC |
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| Subject |
Biochemistry & Molecular Biology; Chemistry
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| Description |
Microarray technology has become an important tool for detection and analysis of nucleic acid targets. Immobilization of modified and unmodified oligonucleotides on epoxy-functionalized glass surfaces is often used in microarray fabrication. Here, we demonstrate a protocol that employs coating of SU-8 (glycidyl ether of bisphenol A) onto glass microslides to obtain high density of epoxy functions for efficient immobilization of aminoalkyl-, thiophosphoryl-, and phosphorylated oligonucleotides with uniform spot morphology. The resulting microarrays exhibited high immobilization (-65%) and hybridization efficiency (30-36%) and were sufficiently stable over a range of temperature and pH conditions. The prominent feature of the protocol is that spots can be visualized distinctly at 0.05 yM probe (a 20-mer oligonucleotide) concentration. The constructed microarrays were subsequently used for detection of base mismatches and bacterial diseases (meningitis and typhoid).
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| Publisher |
AMER CHEMICAL SOCWASHINGTON1155 16TH ST, NW, WASHINGTON, DC 20036 USA
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| Date |
2011-09-20T12:07:06Z
2011-09-20T12:07:06Z 2010 |
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| Type |
Article
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| Identifier |
BIOCONJUGATE CHEMISTRY
1043-1802 http://hdl.handle.net/123456789/13103 |
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| Language |
English
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