Metadata of CSIR Papers
View Archive Info| Field | Value | |
| Creator |
Patel, MK
Solanki, PR Kumar, A Khare, S Gupta, S Malhotra, BD |
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| Subject |
Biophysics; Biotechnology & Applied Microbiology; Chemistry; Electrochemistry; Science & Technology - Other Topics
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| Description |
Meningitis sensor based on nucleic acid probe of Neisseria meningitidis has been fabricated by immobilization of 5'-thiol end labeled single stranded deoxyribonucleic acid probe (ssDNA-SH) onto gold (Au) coated glass electrode. This ssDNA-SH/Au electrode hybridized with the genomic DNA (G-dsDNA/Au) and amplified DNA (PCR-dsDNA/Au) has been characterized using atomic force microscopy (AFM), Fourier transforms infrared spectroscopy (FT-IR) and electrochemical techniques. The ssDNA-SH/Au electrode can specifically detect upto 10-60 ng/mu l of G-dsDNA-SH/Au and PCR-dsDNA-SH/Au of meningitis within 60s of hybridization time at 25 degrees C by cyclic voltammetry (CV) using methylene blue (MB) as electro-active DNA hybridization indicator. The values of sensitivities of the G-dsDNA-SH/Au and PCR-dsDNA-SH/Au electrodes have been determined as 0.0115 mu A/ng cm(-2) and 0.0056 mu A/ng cm(-2), respectively with regression coefficient (R) as 0.999. This DNA bioelectrode is stable for about 4 months when stored at 4 degrees C. (C) 2010 Elsevier B.V. All rights reserved.
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| Publisher |
ELSEVIER ADVANCED TECHNOLOGYOXFORDOXFORD FULFILLMENT CENTRE THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND
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| Date |
2011-09-20T12:07:06Z
2011-09-20T12:07:06Z 2010 |
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| Type |
Article
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| Identifier |
BIOSENSORS & BIOELECTRONICS
0956-5663 http://hdl.handle.net/123456789/13105 |
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| Language |
English
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