Metadata of CSIR Papers
View Archive Info| Field | Value | |
| Creator |
Kapardar, RK
Ranjan, R Puri, M Sharma, R |
|
| Subject |
Biotechnology & Applied Microbiology; Microbiology
|
|
| Description |
Metagenome represent an unlimited resource for discovery of novel genes. Here we report, sequence analysis of a salt tolerant metagenomic clone (6B4) from a pond water metagenomic library. Clone 6B4 had an insert of 2254 bp with G+C composition of 64.06%. DNA sequence from 6B4 showed homology to DNA sequences from pro-teobacteria indicating origin of 6B4 metagenomic insert from a yet uncharacterized proteobacteria. Two encoded proteins from clone 6B4 showed match with ATP-depen-dent Clp protease adaptor protein (ClpS) and phasin, while two truncated encoded proteins showed match with poly-3-hydroxybutyrate synthase and permease. Clp complex is known to play a role in stress tolerance. Expression of ClpS from metagenomic clone is proposed to be responsible for salt tolerance of the metagenomic clone 6B4.
|
|
| Publisher |
SPRINGERNEW YORK233 SPRING ST, NEW YORK, NY 10013 USA
|
|
| Date |
2011-09-20T12:07:09Z
2011-09-20T12:07:09Z 2010 |
|
| Type |
Article
|
|
| Identifier |
INDIAN JOURNAL OF MICROBIOLOGY
0046-8991 http://hdl.handle.net/123456789/13123 |
|
| Language |
English
|
|