Metadata of CSIR Papers
View Archive Info| Field | Value | |
| Creator |
Kumar, A
Balachandran, Y Gupta, S Khare, S Suman |
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| Subject |
Biotechnology & Applied Microbiology
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| Description |
A quick multiplex PCR based detection method was developed for early diagnosis of typhoid using specific genetic markers of S. typhi. Primers of tyv gene, flag gene, viaB gene and ratA gene confirmed the specificity and sensitivity of the PCR. The serum samples of the suspected typhoid patients were taken directly for PCR without culturing and isolating genomic DNA. Overall diagnosis required 2 h which is the least time ever reported for a PCR based methods. The sensitivity of the method is up to 5 fg genomic DNA. The genetic markers are specific and the four pairs of primers give selective amplification and differentiate S. typhi from closely related S. typhimurium.
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| Publisher |
SPRINGERDORDRECHTVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS
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| Date |
2011-09-20T12:07:10Z
2011-09-20T12:07:10Z 2010 |
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| Type |
Article
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| Identifier |
BIOTECHNOLOGY LETTERS
0141-5492 http://hdl.handle.net/123456789/13133 |
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| Language |
English
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