Metadata of CSIR Papers
View Archive Info| Field | Value | |
| Creator |
Patnaik, S
Arif, M Pathak, A Kurupati, R Singh, Y Gupta, KC |
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| Subject |
Science & Technology - Other Topics; Research & Experimental Medicine
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| Description |
Branched polyethylenimine (PEI; 25 kDa) as a nonviral vector exhibits high transfection efficiency and is a potential candidate for efficient gene delivery. However, the cytotoxicity of PEI limits its application in vivo. PEI was ionically interacted with hexametaphosphate, a compact molecule with high anionic charge density, to obtain nanoparticles (PEI-HMP). Nanoparticles were assessed for their efficacy in protecting complexed DNA against nucleases. The intracellular trafficking of nanoparticles was monitored by confocal microscopy. The cytotoxicity and transfection efficiency of PEI-HMP nanoparticles were evaluated in vitro. In vitro transfection efficiency of PEI-HMP (7.7%) was similar to 1.3- to 6.4-folds higher than that of the commercial reagents GenePORTER 2(TM), Fugene(TM), and Superfect(TM). Also, PEI-HMP (7.7%) delivered green fluorescent protein (GFP)-specific small interfering ribonucleic acid (siRNA) in culture cells leading to >80% suppression in GFP gene expression. PEI-HMP nanoparticles protected complexed DNA against DNase for at least 2 hours. A time-course uptake of PEI-HMP (7.7%) nanoparticles showed the internalization of nanoparticles inside the cell nucleus in 2 hours. Thus, PEI-HMP nanoparticles efficiently transfect cells with negligible cytotoxicity and show great promise as nonviral vectors for gene delivery. From the Clinical Editor: Branched polyethylenimine (PEI) as a non-viral vector exhibits high transfection efficiency for gene delivery, but its cytotoxicity limits its applications. PEI hexametaphosphate nanoparticles (PEI-HMP) demonstrated a 1.3-6.4 folds higher transfection rate compared to commercial reagents. Overall, PEI-HMP nanoparticles efficiently transfect cells with negligible cytotoxicity and show great promise as non-viral vectors for gene delivery. (C) 2010 Elsevier Inc. All rights reserved.
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| Publisher |
ELSEVIER SCIENCE BVAMSTERDAMPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
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| Date |
2011-09-20T12:07:10Z
2011-09-20T12:07:10Z 2010 |
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| Type |
Article
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| Identifier |
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
1549-9634 http://hdl.handle.net/123456789/13135 |
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| Language |
English
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