Metadata of CSIR Papers
View Archive Info| Field | Value | |
| Creator |
Tripathi, P
Nair, S Singh, BP Arora, N |
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| Subject |
Biochemistry & Molecular Biology; Endocrinology & Metabolism
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| Description |
Oxidative Stress is implicated in the pathogenesis of asthma, and antioxidant levels are reduced in asthma patients. Previously, glutathione S-transferase (GST) with reduced IgE binding Suppressed oxidative stress and modulated airway inflammation to some extent in mice. GST catalyzes the quenching of reactive oxygen species by reduced glutathione (GSH) and the absence of any one of them may limit antioxidative behavior. This study evaluates the effects of mutated (m) GST with GSH in combination and individually in limiting oxidative stress and inflammatory responses in a mouse model. BALB/c mice were immunized and challenged with ovalbumin. mGST + GSH, or alpha-lipoic acid by inhalation and sacrificed to evaluate The mice were treated with mGST GSH,, inflammation and oxidative stress parameters. Treatment with the mGST + GSH combination showed significantly reduced total cell (p < 0.01) and eosinophil (p < 0.01) counts in BALF compared to other groups. The lung inflammation score was lowest for the mGST + GSH group, along with reduced IL-4 (p < 0.01) and OVA-specific IgE compared to the other treatment groups. Oxidative stress as per the lipid peroxidation and 8-isoprostane level in BALF of mGST + GSH mice was reduced significantly compared to the individual antioxidants. In conclusion, mGST in combination with GSH has a synergistic effect in reducing airway inflammation compared to the individual antioxidants and has potential for the treatment of asthma. (c) 2010 Elsevier Inc. All rights reserved.
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| Publisher |
ELSEVIER SCIENCE INCNEW YORK360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA
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| Date |
2011-09-20T12:07:10Z
2011-09-20T12:07:10Z 2010 |
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| Type |
Article
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| Identifier |
FREE RADICAL BIOLOGY AND MEDICINE
0891-5849 http://hdl.handle.net/123456789/13136 |
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| Language |
English
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