Metadata of CSIR Papers
View Archive Info| Field | Value | |
| Creator |
Patnaik, S
Arif, M Pathak, A Singh, N Gupta, KC |
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| Subject |
Pharmacology & Pharmacy
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| Description |
Branched polyethylenimine (PEI, 25 kDa) was ionically interacted with varying amount of alginic acid to block different proportion (2.6-5.7%) of amines in PEI to form a series of nanocomposites, PEI-Al. These nanocomposites, upon interaction with DNA, protected it against DNase I. Among various complexes evaluated, PEI-Al(4.8%)/DNA displayed the highest transfection efficiency in HEK293, COS-1 and HeLa cells that was similar to 2-8-folds higher than Superfect (TM), Fugene (TM), PEI (750kDa)-Al(6.26%) and PEI alone. The projected nanocomposites were nearly non-toxic to cells in vitro.Furthermore, the concentration of PEI-Al(4.8%) needed to deliver GFP-specific siRNA in COS-1 cells was 20 times lower than PEI (750 kDa)Al(6.26%). Intracellular trafficking of PEI-Al(4.8%) with or without complexed DNA in HeLa cells shows that both appear in the nucleus after 1 h. (c) 2009 Elsevier B.V. All rights reserved.
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| Publisher |
ELSEVIER SCIENCE BVAMSTERDAMPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
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| Date |
2011-09-20T12:07:12Z
2011-09-20T12:07:12Z 2010 |
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| Type |
Article
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| Identifier |
INTERNATIONAL JOURNAL OF PHARMACEUTICS
0378-5173 http://hdl.handle.net/123456789/13149 |
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| Language |
English
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