Metadata of CSIR Papers
View Archive Info| Field | Value | |
| Creator |
Tiwari, JK
Poonam Sarkar, D Pandey, SK Gopal, J Kumar, SR |
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| Subject |
Biotechnology & Applied Microbiology; Plant Sciences
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| Description |
Interspecific potato somatic hybrids between Solanum tuberosum L. (di)haploid C-13 and 1 endosperm balance number non-tuberous wild species S. etuberosum Lindl. were produced by protoplasts electrofusion. The objective was to transfer virus resistance from this wild species into the cultivated potatoes. Post-fusion products were cultured in VKM medium followed by regeneration of calli in MS(13) K medium at 20A degrees C under a 16-h photoperiod, and regenerants were multiplied on MS medium. Twenty-one somatic hybrids were confirmed by RAPD, SSR and cytoplasm (chloroplast/mitochondria) type analysis possessing species-specific diagnostic bands of corresponding parents. Tetraploid nature of these somatic hybrids was determined through flow cytometry analysis. Somatic hybrids showed intermediate phenotypes (plant, leaves and floral morphology) to their parents in glass-house grown plants. All the somatic hybrids were male-fertile. ELISA assay of somatic hybrids after artificial inoculation of Potato virus Y (PVY) infection reveals high PVY resistance.
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| Publisher |
SPRINGERDORDRECHTVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS
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| Date |
2011-09-20T12:08:54Z
2011-09-20T12:08:54Z 2010 |
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| Type |
Article
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| Identifier |
PLANT CELL TISSUE AND ORGAN CULTURE
0167-6857 http://hdl.handle.net/123456789/13550 |
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| Language |
English
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