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Field Value
 
Creator Negi, A
Rana, T
Kumar, Y
Ram, R
Hallan, V
Zaidi, AA
 
Subject Biochemistry & Molecular Biology; Plant Sciences
 
Description A survey was undertaken in the temperate fruit growing regions of Himachal Pradesh (HP) and Jammu & Kashmir (J&K). Apple stem grooving virus (ASGV), a Capillovirus, was detected in different cultivars of apple, nectarines, plum, cherry, quince and apricot by double antibody sandwich ELISA (DAS-ELISA). The coat protein (CP) gene sequence of an amplicon produced by RT-PCR, confirmed the association of ASGV in apple cultivar Starkrimson, collected from Himachal Pradesh. The CP of Indian ASGV isolate shared 100 % sequence identity with a Brazilian isolate (AF438409). Sequence analysis by Recombination Detection Program (RDP2) indicated no recombination event for the Indian isolate. However, recombination was detected in Chinese, Korean and Citrus tatter leaf virus-Taiwan (CTLV) strains of ASGV. The study describes first report of ASGV infection in India and characterization of its CP gene.
 
Publisher SOC PLANT BIOCHEM BIOTECHNEW DELHIDIV BIOCHEM, INDIAN AGRICULTURAL RES INST, NEW DELHI 110 012, INDIA
 
Date 2011-09-20T12:08:58Z
2011-09-20T12:08:58Z
2010
 
Type Article
 
Identifier JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY
0971-7811
http://hdl.handle.net/123456789/13584
 
Language English