Cellobiase from Termitomyces clypeatus: activity and secretion in presence of glycosylation inhibitors
Metadata of CSIR Papers
View Archive Info| Field | Value | |
| Title |
Cellobiase from Termitomyces clypeatus: activity and secretion in presence of glycosylation inhibitors
|
|
| Creator |
Mukherjee, S
Chowdhury, S Ghorai, S Pal, S Khowala, S |
|
| Subject |
Biotechnology & Applied Microbiology
|
|
| Description |
In presence of the glycosylation inhibitors, 2-deoxy-D-glucose (1 mg/ml), tunicamycin (30 mu g/ml), 1-deoxynojirimycin (30 mu g/ml) and D-glucono-delta-lactone (1 mg/ml), total cellobiase activity, in the extracellular, intracellular and cell bound fractions, of the fungus Termitomyces clypeatus grown in 20 ml cellobiose medium (1%, w/v) increased by 50-, 1.8-, 2.4-, 1.3-fold, respectively, with respect to control medium (16.3 U). The inhibitors also stimulated secretion of 95% of the total protein in culture medium, except D-glucono-delta-lactone which released 60% of the total protein. 2-Deoxy-D-glucose (1 mg/ml) led to production of extracellular cellobiase up to 40 U/ml, whereas in absence of the inhibitors only 0.59 U/ml enzyme was detected.
|
|
| Publisher |
SPRINGERDORDRECHTVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS
|
|
| Date |
2011-09-20T12:12:08Z
2011-09-20T12:12:08Z 2006 |
|
| Type |
Article
|
|
| Identifier |
BIOTECHNOLOGY LETTERS
0141-5492 http://hdl.handle.net/123456789/14137 |
|
| Language |
English
|
|