High-yielding plasmid extraction method from acidophilic heterotrophic bacteria of the genus Acidiphilium
Metadata of CSIR Papers
View Archive Info| Field | Value | |
| Title |
High-yielding plasmid extraction method from acidophilic heterotrophic bacteria of the genus Acidiphilium
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| Creator |
Singh, SK
Banerjee, PC |
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| Subject |
Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Analytical
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| Description |
Plasmid yield from Acidiphilium strains always had been poor following various standard methods. We adopted some simple modifications in the alkaline lysis procedure to get a better yield of plasmid from these bacteria. An approximately 10- to 20-fold increase in the plasmid yield was achieved when harvested Acidiphilium cells were preincubated 16-20 h at pH 6 in nitrogen-free medium. Another independent approach showed that freezing (-18 to -20 degrees C) of the harvested cells initially and at two subsequent steps in the alkaline lysis procedure of plasmid DNA extraction improved the yield further by 1.5- to 3-fold. The combination of these changes yielded at least 15- to 30-fold more plasmid from various Acidiphilium strains as compared with standard methods. (c) 2006 Elsevier Inc. All rights reserved.
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| Publisher |
ACADEMIC PRESS INC ELSEVIER SCIENCESAN DIEGO525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA
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| Date |
2011-09-20T12:12:08Z
2011-09-20T12:12:08Z 2006 |
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| Type |
Article
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| Identifier |
ANALYTICAL BIOCHEMISTRY
0003-2697 http://hdl.handle.net/123456789/14139 |
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| Language |
English
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