Leishmania donovani infection of human myeloid dendritic cells leads to a Th1 response in CD4(+) T cells from healthy donors and patients with kala- azar
Metadata of CSIR Papers
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| Title |
Leishmania donovani infection of human myeloid dendritic cells leads to a Th1 response in CD4(+) T cells from healthy donors and patients with kala- azar
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| Creator |
Ghosh, M
Mandal, L Maitra, S Rakshit, S Paul, K Bagchi, J Ganguly, D Pal, C Bandyopadhyay, S |
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| Subject |
Infectious Diseases
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| Description |
The role played by dendritic cells (DCs) in Leishmania donovani infection is poorly understood. Here, we report that L. donovani amastigotes efficiently infect human peripheral-blood monocyte-derived DCs. Opsonization with normal human serum enhanced the infectivity of amastigotes and promastigotes only marginally. Surface attachment versus internalization was distinguished by incubation of DCs with live, fluorescein isothiocyanate-labeled parasites, followed by quenching with crystal violet. Infection with amastigotes was accompanied by DC maturation, as was evident from the up-regulation of maturation-associated cell-surface markers, the nuclear translocation of RelB, and the release of cytokines. Amastigote-primed DCs produced inflammatory cytokines in response to subsequent treatment with interferon-gamma or anti-CD40 monoclonal antibody. When cocultured, amastigote-infected DCs induced T helper cell type 1 (Th1) responses both in naive allogeneic CD4(+) T cells and in autologous CD4(+) T cells from patients with kala-azar and up-regulated the expression of T-bet. Our data reveal that infection with L. donovani amastigotes induces a Th1 cytokine milieu in both DCs and T cells.
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| Publisher |
UNIV CHICAGO PRESSCHICAGO1427 E 60TH ST, CHICAGO, IL 60637-2954 USA
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| Date |
2011-09-20T12:12:11Z
2011-09-20T12:12:11Z 2006 |
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| Type |
Article
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| Identifier |
JOURNAL OF INFECTIOUS DISEASES
0022-1899 http://hdl.handle.net/123456789/14159 |
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| Language |
English
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