A heparin-binding membrane protein from goat spermatozoa immobilizes spermatozoa in the presence of complement
Metadata of CSIR Papers
View Archive Info| Field | Value | |
| Title |
A heparin-binding membrane protein from goat spermatozoa immobilizes spermatozoa in the presence of complement
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| Creator |
Mor, V
Das, T Ray, K Chatterjee, T |
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| Subject |
Obstetrics & Gynecology; Reproductive Biology
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| Description |
Objective: To characterize the major membrane protein of goat spermatozoa. Design: Basic research. Setting: Samples collected from local slaughterhouse and study conducted in an academic research environment. Patient(s): Goat epididymal tissue. Intervention(S): Goat epididymal tissues were collected immediately after slaughter and the spermatozoa were isolated within 2 hours. Sperm immobilization test was performed with motile spermatozoa at 32 degrees C within 3-4 hours of collection and isolation of the cells. Main Outcome Measure(s): The heparin-binding sperm membrane protein (HBSM) of goat is insensitive to trypsin and its antisera immobilize spermatozoa in presence of complement. Result(s): Forty-two percent of membrane protein could be extracted with 0.25% (wt/vol) 3-[(3-Cholamidopropyl) dimethylammoniol-1-propanesulfonate (CHAPS) from the isolated sperm membrane. By heparin-affinity chromatography. 46% of the extracted protein was recovered. Positive hybridization with radiolabeled heparin on western transfer confirmed the heparin-binding property of the protein (HBSM). Heparin binding to HBSM is an ionic bondage and can be disrupted by 1 M NaCl, as revealed by 86% recovery of the radiolabeled heparin in trichloroacetic acid-precipitated supernatant of [I-125] heparin-HBSM conjugate. Heparin-binding sperm membrane protein is localized at the anterior region of the spermatozoal head. No detectable proteolytic fragment of HBSM was detected after limited digestion by trypsin. Heparin-binding sperm membrane protein antisera (1: 10,000 titer) developed from rabbit did not recognize the denatured protein. The antisera inhibited spermatozoal motility in a complement-dependent manner. Conclusion(s): We suggest that the heparin-binding motif of the spermatozoal membrane protein might be required in modulation of the spermatozoal motility.
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| Publisher |
ELSEVIER SCIENCE INCNEW YORK360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA
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| Date |
2011-09-20T12:12:16Z
2011-09-20T12:12:16Z 2006 |
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| Type |
Article
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| Identifier |
FERTILITY AND STERILITY
0015-0282 http://hdl.handle.net/123456789/14200 |
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| Language |
English
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