9-O-acetylated sialoglycoproteins: novel molecules in childhood acute lymphoblastic leukaemia (ALL)
Metadata of CSIR Papers
View Archive Info| Field | Value | |
| Title |
9-O-acetylated sialoglycoproteins: novel molecules in childhood acute lymphoblastic leukaemia (ALL)
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| Creator |
Ghosh, S
Bandyopadhyay, S Pal, S Sinha, D Mallick, A Chatterjee, M Mandal, C Mandal, C |
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| Subject |
Multidisciplinary Sciences
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| Description |
The expanding field of glycobiology deals with the investigation of structure-function relationships of carbohydrate molecules enabling better understanding of their diverse role in biological systems ranging from inflammation, immunity, cell development and cancers. Attention is currently focused on the importance of glycosylation in disease. Neoplastic transformation causes changes in cell surface architecture, most notably, aberrant sialylation. Childhood Acute Lymphoblastic Leukaemia (ALL). a clonal lymphoproliferative disorder, is characterized by marked overproduction of lymphoblasts. Although it is highly responsive to chemotherapy, residual blasts are responsible for disease persistence, eventually resulting in relapse. This is defined as minimal residual disease (MRD) and is a major challenge in leukaemia research. Therefore, identification of disease-specific cell surface biomarkers and establishment of reliable techniques may be useful for diagnosis. prognosis and prediction of MRD. Ongoing research in our laboratory is mainly engaged in studying the relationship between carbohydrates and the immune response. We have firmly established a novel biomarker namely 9-O-acetylated sialoglycoproteins (Neu5,9Ac(2)-GPs) on lymphoblasts of children with ALL by exploiting the selective affinity of Achatinin-H, a lectin that specifically binds to N-acetl-9-O-acetylneuraminic acid-alpha 2-6-GalNAc. Achatinin-1-1 was subsequently used to develop a lymphoproliferation assay for ALL where the extent of lymphoproliferation induced by the lectin served as an index for monitoring the disease-status in ALL. Accordingly, this article encompasses our ongoing research on ALL that is broadly focused on (i) identification of novel antigens namely, Neu5,9Ac(2)-GPs as biomarkers on lymphoblasts. (ii) development of a lymphoproliferation assay for monitoring the disease status (iii) identification of antibodies directed against Neu5,9Ac(2)-GPs in children with ALL (iv) employing these purified disease-specific antibodies in developing an ELISA based diagnostic method and (v) studying the biological role of these biomarkers (antigen and antibodies) in escaping immune surveillance.
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| Publisher |
NATL ACAD SCIENCES INDIAALLAHABAD5 LAJPATRAI RD, ALLAHABAD 211002, INDIA
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| Date |
2011-09-20T12:12:18Z
2011-09-20T12:12:18Z 2006 |
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| Type |
Article
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| Identifier |
NATIONAL ACADEMY SCIENCE LETTERS-INDIA
0250-541X http://hdl.handle.net/123456789/14211 |
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| Language |
English
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