Extracellular trehalose utilization by Saccharomyces cerevisiae
Metadata of CSIR Papers
View Archive Info| Field | Value | |
| Title |
Extracellular trehalose utilization by Saccharomyces cerevisiae
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| Creator |
Basu, A
Bhattacharyya, S Chaudhuri, P Sengupta, S Ghosh, AK |
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| Subject |
Biochemistry & Molecular Biology; Biophysics
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| Description |
Two haploid strains of Saccharomyces cerevisiae viz. MAT alpha and MATa were grown in glucose and trehalose medium and growth patterns were compared. Both strains show similar growth, except for an extended lag phase in trehalose grown cells. In both trehalose grown strains increase in activities of both extracellular trehalase activities and simultaneous decrease in extracellular trehalose level was seen. This coincided with a sharp increase in extracellular glucose level and beginning of log phase of growth. Alcohol production was also observed. Secreted trehalase activity was detected, in addition to periplasmic activity. It appeared that extracellular trehalose was hydrolyzed into glucose by extracellular trehalase activity. This glucose was utilized by the cells for growth. The alcohol formation was due to the fermentation of glucose. Addition of extracellular trehalase caused reduction in the lag phase when grown in trehalose medium, supporting our hypothesis of extracellular utilization of trehalose. (c) 2005 Elsevier B.V. All rights reserved.
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| Publisher |
ELSEVIER SCIENCE BVAMSTERDAMPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
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| Date |
2011-09-20T12:12:18Z
2011-09-20T12:12:18Z 2006 |
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| Type |
Article
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| Identifier |
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
0304-4165 http://hdl.handle.net/123456789/14214 |
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| Language |
English
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