Purification, crystallization and preliminary X-ray structural studies of a 7.2 kDa cytotoxin isolated from the venom of Daboia russelli russelli of the Viperidae family
Metadata of CSIR Papers
View Archive Info| Field | Value | |
| Title |
Purification, crystallization and preliminary X-ray structural studies of a 7.2 kDa cytotoxin isolated from the venom of Daboia russelli russelli of the Viperidae family
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| Creator |
Choudhury, SR
Gomes, A Dattagupta, JK Sen, U |
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| Subject |
Biochemical Research Methods; Biochemistry & Molecular Biology; Biophysics; Crystallography
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| Description |
A cytotoxin (MW 7.2 kDa) from Indian Russell's viper (Daboia russelli russelli) venom possessing antiproliferative activity, cardiotoxicity, neurotoxicity and myotoxicity has been purified, characterized and crystallized. The crystals belong to the tetragonal space group P4(1), with unit-cell parameters a = b = 47.94, c = 50.2 angstrom. Larger crystals, which diffracted to 1.5 angstrom, were found to be twinned; diffraction data were therefore collected to 2.93 angstrom resolution using a smaller crystal. Molecular-replacement calculations identified two molecules of the protein in the asymmetric unit, which is in accordance with the calculated V-M value.
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| Publisher |
BLACKWELL PUBLISHINGOXFORD9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLAND
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| Date |
2011-09-20T12:12:19Z
2011-09-20T12:12:19Z 2006 |
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| Type |
Article
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| Identifier |
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS
1744-3091 http://hdl.handle.net/123456789/14224 |
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| Language |
English
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