Induction of host protective th1 immune response by chemokines in Leishmania donovani-infected BALB/c mice
Metadata of CSIR Papers
View Archive Info| Field | Value | |
| Title |
Induction of host protective th1 immune response by chemokines in Leishmania donovani-infected BALB/c mice
|
|
| Creator |
Dey, R
Majumder, N Majumdar, SB Bhattacharjee, S Banerjee, S Roy, S Majumdar, S |
|
| Subject |
Immunology
|
|
| Description |
The resolution from leishmanial infection is dependent on the coordinated interactions between the components of the cell mediated immune system and the activation of T-cell population into appropriate cytokine production and the activation of macrophages. Earlier reports established that C-C chemokines particularly macrophage inflammatory protein (MIP)-1 alpha and macrophage chemoattractant protein (MCP)-1 restrict the parasitic burden via the regulation of impaired protein kinase C (PKC) signalling and induction of free-radical generation in murine leishmaniasis. This study explored the role of MIP-1 alpha and MCP-1 in the induction of T helper 1 (Th1) immune response and suppression of T helper 2 (Th2) response in Leishmania donovani-infected BALB/c mice. These chemokines induced the known pro-inflammatory cytokine interleukin (IL)-12 secretion and inhibited the secretion of anti-inflammatory cytokines IL-10 and transforming growth factor-beta in infected macrophages. Impaired antigen presentation capability of infected macrophages was also restored by the chemokine treatment. C-C chemokine treatment resulted in reduced levels of mRNA expression of IL-10, but increased levels of mRNA expression of IL-12p40, interferon (IFN)-gamma, tumour necrosis factor-alpha and inducible nitric oxide synthase in both liver mononuclear cells as well as in splenocytes, reflecting a switch of CD4(+) differentiation from Th2 to Th1. Flow cytometric analysis of infected spleen cells suggested that C-C chemokine treatment enhances the CD4(+) T cells to produce increased levels of IFN-gamma. These studies hypothesize a promising immuno-prophylactic effect of chemokines against leishmaniasis by induction of Th1 cytokine release imparting a long-term resistance.
|
|
| Publisher |
BLACKWELL PUBLISHINGOXFORD9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLAND
|
|
| Date |
2011-09-20T12:12:26Z
2011-09-20T12:12:26Z 2007 |
|
| Type |
Article
|
|
| Identifier |
SCANDINAVIAN JOURNAL OF IMMUNOLOGY
0300-9475 http://hdl.handle.net/123456789/14271 |
|
| Language |
English
|
|