Human intestinal epithelial cell cytokine mRNA responses mediated by NF-kappa B are modulated by the motility and adhesion process of vibrio cholerae
Metadata of CSIR Papers
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| Title |
Human intestinal epithelial cell cytokine mRNA responses mediated by NF-kappa B are modulated by the motility and adhesion process of vibrio cholerae
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| Creator |
Bandyopadhaya, A
Sarkar, M Chaudhuri, K |
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| Subject |
Biochemistry & Molecular Biology; Cell Biology
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| Description |
Vibro cholerae, the etiological agent of cholera, colonizes the small intestine, produces an enterotoxin and causes acute inflammatory response at intestinal epithelial surface; the signals for such induction are still unknown. We determined the mRNA expression of proinflammatory and anti-inflammatory cytokines in Int407 cells following infection with V cholerae or its mutants by semi-quantitaive and quantitative real-time RT-PCR. V cholerae induces the coordinated expression and up-regulation of IL-1 alpha, IL-6, GM-CSF and MCP-1 and down-regulation of TGF-beta in Int407 cells. While the pathogenecity of V choterae was found to be a possible determinant in modulation of IL-1 alpha and TGF-beta, both IL-6 and MCP-1 OmpU might modulate induction. Significant reduction in IL-la, GM-CSF and MCP-1 mRNA expression was observed upon infection with the less motile and less adherent strain 0395YN. This association is supported by the absence of nuclear translocation of NF-kappa B (p50 subunit) upon infection with 0395YN in contrast to wild-type. Moreover, TPCK treatment prior to V cholerae infection indicated that proinflammatory cytokine gene expression in Int407 cells is NF-kappa B mediated. Thus, V cholerae induces proinflammatory cytokine response in Int407 cells, which is mediated by NF-kappa B and is modulated, in part, by adherence or motility of this organism. (c) 2007 Elsevier Ltd. All rights reserved.
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| Publisher |
PERGAMON-ELSEVIER SCIENCE LTDOXFORDTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND
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| Date |
2011-09-20T12:12:28Z
2011-09-20T12:12:28Z 2007 |
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| Type |
Article
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| Identifier |
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
1357-2725 http://hdl.handle.net/123456789/14284 |
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| Language |
English
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