Differential regulation of interchromosomal copies of ToxR-induced genes
Metadata of CSIR Papers
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| Title |
Differential regulation of interchromosomal copies of ToxR-induced genes
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| Creator |
Nag, S
Chaudhuri, K |
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| Subject |
Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Immunology; Microbiology
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| Description |
In Vibrio cholerae, ToxR transcriptionally activates a number of virulence genes in response to various environmental signals. In the present study, transcription profiling by macroarray was carried out with 13 pairs of genes, one copy of which is present in each chromosome under ToxR-inducing (pH 6.5, osmolarity 66 mmol/L, 30 degrees C) and ToxR-repressing (pH 8.5, osmolarity 300 mmol/L, 37 degrees C) conditions followed by high pH (8.5) and low pH (6.5) conditions to eliminate pH effect. The genes dacAII, tagEII, secDH, pmmI, pmmIl, and immII showed increased expression in the ToxR-inducing conditions, but not at low pH, suggesting that the expression of these genes might be regulated by ToxR. The expression of pmmII, dacAII, tagEII, secDII, and immII genes decreased significantly in the toxR insertion mutant as determined by RT-PCR, whereas the expression of the chromosome I copy of pmm. increased in toxR mutant compared with wild-type cells. Thus, the chromosome 11 copy of these genes, which show increased expression under ToxR-inducing conditions, are all regulated by ToxR in V. cholerae, whereas the chromosome I copy of pmm may be regulated by other factors under ToxR-inducing conditions.
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| Publisher |
NATL RESEARCH COUNCIL CANADA-N R C RESEARCH PRESSOTTAWABUILDING M 55, OTTAWA, ON K1A 0R6, CANADA
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| Date |
2011-09-20T12:12:28Z
2011-09-20T12:12:28Z 2007 |
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| Type |
Article
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| Identifier |
CANADIAN JOURNAL OF MICROBIOLOGY
0008-4166 http://hdl.handle.net/123456789/14285 |
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| Language |
English
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