Viper and cobra venom neutralization by beta-sitosterol and stigmasterol isolated from the root extract of Pluchea indica Less. (Asteraceae)
Metadata of CSIR Papers
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| Title |
Viper and cobra venom neutralization by beta-sitosterol and stigmasterol isolated from the root extract of Pluchea indica Less. (Asteraceae)
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| Creator |
Gomes, A
Saha, A Chatterjee, I Chakravarty, AK |
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| Subject |
Plant Sciences; Chemistry, Medicinal; Pharmacology & Pharmacy
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| Description |
We reported previously that. the methanolic root extract of the Indian medicinal plant Pluehea indica Less. (Asteraceae) could neutralize viper venom-induced action [Alam, M.I., Auddy, B., Gomes, A., 1996. Viper venom neutralization by Indian medicinal plant (Hemidesmus indicus and P. indica) root extracts. Phytother. Res. 10, 58-61]. The present study reports the neutralization of viper and cobra venom by beta-sitosterol and stigmasterol isolated from the root extract of P. indica Less. (Asteraceae). The active fraction (containing the major compound beta-sitosterol and the minor compound stigmasterol) was isolated and purified by silica gel column chromatography and the structure was determined using spectroscopic analysis (EIMS, H-1 NMR, C-13 NMR). Anti-snake venom activity was studied in experimental animals. The active fraction was found to significantly neutralize viper venom-induced lethal, hemorrhagic, defibrinogenation, edema and PLA(2) activity. Cobra venom-induced lethality, cardiotoxicity, neurotoxicity, respiratory changes and PLA(2) activity were also antagonized by the active component. It potentiated commercial snake venom antiserum action against venom-induced lethality in male albino mice. The active fraction could antagonize venom-induced changes in lipid peroxidation and superoxide dismutase activity. This study suggests that beta-sitosterol and stigmasterol may play an important role, along with antiserum, in neutralizing snake venominduced actions. (c) 2007 Elsevier GrnbH. All rights reserved.
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| Publisher |
ELSEVIER GMBH, URBAN & FISCHER VERLAGJENAOFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY
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| Date |
2011-09-20T12:12:28Z
2011-09-20T12:12:28Z 2007 |
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| Type |
Article
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| Identifier |
PHYTOMEDICINE
0944-7113 http://hdl.handle.net/123456789/14287 |
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| Language |
English
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