Specific binding and self-structure induction to poly(A) by the cytotoxic plant alkaloid sanguinarine
Metadata of CSIR Papers
View Archive Info| Field | Value | |
| Title |
Specific binding and self-structure induction to poly(A) by the cytotoxic plant alkaloid sanguinarine
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| Creator |
Giri, P
Kumar, GS |
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| Subject |
Biochemistry & Molecular Biology; Biophysics
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| Description |
The cytotoxic plant alkaloid sanguinarine was found to bind preferentially and strongly to single stranded poly(A) with an association constant (K-a) in the range 3.6-4.6 x 10(6) M(-1)in comparison to several nucleic acids. The binding induced unique self-structure formation in poly(A) that showed cooperative melting transition in circular dichroism, absorbance, and differential scanning calorimetry studies. The alkaloid binding was characterized to be intercalation as revealed from fluorescence quenching experiments and was predominantly enthalpy driven as revealed from isothermal titration calorimetry. Sanguinarine is the first and only natural product so far known to induce a self-structure formation in poly(A). (C) 2007 Elsevier B.V. All rights reserved.
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| Publisher |
ELSEVIER SCIENCE BVAMSTERDAMPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
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| Date |
2011-09-20T12:12:29Z
2011-09-20T12:12:29Z 2007 |
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| Type |
Article
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| Identifier |
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
0304-4165 http://hdl.handle.net/123456789/14292 |
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| Language |
English
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