Creating a macromolecular receptor by affinity imprinting
Metadata of CSIR Papers
View Archive Info| Field | Value | |
| Title |
Creating a macromolecular receptor by affinity imprinting
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| Creator |
Vaidya, AA
Lele, BS Kulkarni, MG Mashelkar, RA |
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| Subject |
Polymer Science
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| Description |
A molecularly imprinted polymeric receptor for trypsin was synthesized by employing a novel technique that is a combination of affinity separation and molecular imprinting. An enzyme-inhibitor complex of trypsin and N-acryloyl para-aminobenzamidine was polymerized with acrylamide and N,N'-methylene bis-acrylamide. Template trypsin was extracted out to obtain an affinity-imprinted polymer. Control experiments were performed to demonstrate the synergistic affinity-imprinting effect. The percentage of crosslinker used was the crucial factor in determining the imprinting efficacy of the polymers. Imprinted polymer containing 50% crosslinker exhibited a linear Scatchard plot. Unlike non-imprinted gel, the receptor exhibited almost exclusive recognition of trypsin in an individual batch experiment as well as from a mixture of trypsin and chymotrypsin. (C) 2001 John Wiley & Sons, Inc.
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| Publisher |
JOHN WILEY & SONS INCNEW YORK605 THIRD AVE, NEW YORK, NY 10158-0012 USA
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| Date |
2011-09-24T08:33:37Z
2011-09-24T08:33:37Z 2001 |
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| Type |
Article
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| Identifier |
JOURNAL OF APPLIED POLYMER SCIENCE
0021-8995 http://hdl.handle.net/123456789/24051 |
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| Language |
English
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