Production of beta-carotene by a mutant of Rhodotorula glutinis
Metadata of CSIR Papers
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| Title |
Production of beta-carotene by a mutant of Rhodotorula glutinis
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| Creator |
Bhosale, PB
Gadre, RV |
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| Subject |
Biotechnology & Applied Microbiology
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| Description |
Wild strains of Rhodotorula glutinis and R. rubra were investigated concerning their carotenoid production, proportion of beta -carotene and cell mass yield. R. glutinis NCIM 3353 produced 2.2 mg carotenoid/l in 72 h; and the amount of beta -carotene was 14% (w,w) of the total carotenoid content (17 mug/g cell dry weight). It was subjected to mutagenesis using UV radiation for strain improvement. Out of 2,051 isolates screened, the yellow coloured mutant 32 produced 120-fold more beta -carotene (2,048 mug/g cell dry weight) than the parent culture in 36 h, which was 82% (w/w) of the total carotenoid content. Mutant 32 was grown on different carbon and nitrogen sources. The best yield of beta -carotene (33 +/-3 mg/l) was obtained when glucose and yeast extract were supplied as carbon and nitrogen sources, respectively. Divalent cation salts further increased the total carotenoid content (66 +/-2 mg/l) with beta -carotene as the major component (55 +/-2%, w/w).
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| Publisher |
SPRINGER-VERLAGNEW YORK175 FIFTH AVE, NEW YORK, NY 10010 USA
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| Date |
2011-09-24T08:34:37Z
2011-09-24T08:34:37Z 2001 |
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| Type |
Article
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| Identifier |
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
0175-7598 http://hdl.handle.net/123456789/24053 |
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| Language |
English
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