Assessment of genetic variability and strain identification of Xanthomonas oryzae pv. oryzae using RAPD-PCR and IS1112-based PCR
Metadata of CSIR Papers
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| Title |
Assessment of genetic variability and strain identification of Xanthomonas oryzae pv. oryzae using RAPD-PCR and IS1112-based PCR
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| Creator |
Gupta, VS
Rajebhosale, MD Sodhi, M Singh, S Gnanamanickam, SS Dhaliwal, HS Ranjekar, PK |
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| Subject |
Multidisciplinary Sciences
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| Description |
Sixteen isolates of Xanthomonas oryzae pv, oryzae (X.o. pv. oryzae) representing different geographical locations in India along with 2 isolates from the Philippines were analysed using polymorphic RAPDs. The primers OPA-03, OPA-04, OPA-10, OPA-11, OPK-7, OPK-12 and OPK-17 generated simple, specific and reproducible fingerprint patterns, indicating usefulness of RAPD markers in differentiating X, o, pv. oryzae isolates. At a similarity index of 0.5 the isolates were grouped into only 2 clusters, suggesting that these primers are not very efficient in grouping isolates. Primers PJEL1 and PJEL2, used in insertion sequence IS1112-based PCR, also generated specific and reproducible fingerprint patterns for the same set of the X,o, pv, oryzae isolates. Based on the RAPD-PCR (seven primers) and IS1112-PCR (two primers) data, at a similarity of 0.57, sixteen out of 18 isolates were grouped into 5 different clusters and 2 isolates were loosely grouped with them. The data using RAPD-PCR and IS1112-based PCR approaches revealed their potential in rapid identification of isolates, in assessment of genetic variation in the Indian pathogen population and in generating unique DNA fragments specific to 8 isolates of X.o. pv. oryzae.
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| Publisher |
CURRENT SCIENCE ASSNBANGALOREC V RAMAN AVENUE, PO BOX 8005, BANGALORE 560 080, INDIA
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| Date |
2011-09-24T08:38:07Z
2011-09-24T08:38:07Z 2001 |
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| Type |
Article
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| Identifier |
CURRENT SCIENCE
0011-3891 http://hdl.handle.net/123456789/24060 |
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| Language |
English
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