Active site characterization of the single endo-polygalacturonase produced by Fusarium moniliforme NCIM 1276
Metadata of CSIR Papers
View Archive Info| Field | Value | |
| Title |
Active site characterization of the single endo-polygalacturonase produced by Fusarium moniliforme NCIM 1276
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| Creator |
Niture, SK
Pant, A Kumar, AR |
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| Subject |
Biochemistry & Molecular Biology
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| Description |
Fusarium moniliforme NCIM 1276 isolated from a tropical mangrove ecosystem produces a single extracellular endo polygalacturonase with an M-r of 38 kDa and a carbohydrate: content of 4%. It has an alkaline pI of 8.1. The K-m is 0.12 mg.mL(-1), V-max is 111.1 mu mol.min(-1).mg(-1) and the k(cat) is 4200 min(-1). It has a pH optimum of 4.8. Kinetic and fluorescence data show that tryptophan is involved in binding. An arginine residue at or near the active site may be involved in extended binding of the substrate. A carboxylate and a histidine residue are involved in catalysis. These data are discussed with reference to current literature.
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| Publisher |
BLACKWELL SCIENCE LTDOXFORDP O BOX 88, OSNEY MEAD, OXFORD OX2 0NE, OXON, ENGLAND
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| Date |
2011-09-24T09:14:12Z
2011-09-24T09:14:12Z 2001 |
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| Type |
Article
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| Identifier |
EUROPEAN JOURNAL OF BIOCHEMISTRY
0014-2956 http://hdl.handle.net/123456789/24132 |
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| Language |
English
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