Immobilization of alkaline protease from Conidiobolus macrosporus for reuse and improved thermal stability
Metadata of CSIR Papers
View Archive Info| Field | Value | |
| Title |
Immobilization of alkaline protease from Conidiobolus macrosporus for reuse and improved thermal stability
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| Creator |
Tanksale, A
Chandra, PM Rao, M Deshpande, V |
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| Subject |
Biotechnology & Applied Microbiology
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| Description |
Alkaline protease from Conidiobolus macrosporus was immobilized on polyamide using glutaraldehyde as a bifunctional agent. The immobilized enzyme was optimally active at a higher temperature of 50 degreesC than the free enzyme (40 degreesC ) and showed a ten-fold increased thermostability at 60 degreesC compared to that of the free enzyme. The efficiency of immobilization was 58% under the optimal conditions of pH and temperature. There was a 14-fold decrease in the K-m of immobilized enzyme compared to the free enzyme. The immobilized enzyme was fully active even after twenty-two cycles of repeated use. It retained 80% activity at 50 degreesC in presence of 8 M urea exhibiting its stability to the denaturant and was compatible with several commercial detergents.
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| Publisher |
KLUWER ACADEMIC PUBLDORDRECHTSPUIBOULEVARD 50, PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS
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| Date |
2011-09-24T09:33:14Z
2011-09-24T09:33:14Z 2001 |
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| Type |
Article
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| Identifier |
BIOTECHNOLOGY LETTERS
0141-5492 http://hdl.handle.net/123456789/24170 |
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| Language |
English
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