Synthesis of PHB by recombinant E. coli harboring an approximately 5 kb genomic DNA fragment from Streptomyces aureofaciens NRRL 2209
Metadata of CSIR Papers
View Archive Info| Field | Value | |
| Title |
Synthesis of PHB by recombinant E. coli harboring an approximately 5 kb genomic DNA fragment from Streptomyces aureofaciens NRRL 2209
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| Creator |
Ramachander, TVN
Rohini, D Belhekar, A Rawal, SK |
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| Subject |
Biochemistry & Molecular Biology
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| Description |
An similar to 5.0 kb Sau3A I genomic DNA fragment from Streptomyces aureofaciens NRRL 2209 was cloned in a plasmid vector and introduced into Escherichia coli. The recombinant E coli. accumulated polyhydroxyalkanoates (PHAs) as cytoplasmic inclusions. The accumulated PHA was identified as the isotactic homopolymer of PHB with a molecular weight of 2.85 x 10(5). Purified PHB granules were spherical with an average size of 1.1 mum and of stable configuration. DSC thermogram suggested high crystalline nature of the polymer. Maximum thermal degradation of the biopolymer occurred between 250 and 340 degreesC. Recombinant E coli cells preferentially utilized glycerol as the carbon source and accumulated 25-28 times more PHB than the native S. aureofaciens. (C) 2002 Elsevier Science B.V. All rights reserved.
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| Publisher |
ELSEVIER SCIENCE BVAMSTERDAMPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
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| Date |
2011-09-24T09:40:14Z
2011-09-24T09:40:14Z 2002 |
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| Type |
Article
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| Identifier |
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
0141-8130 http://hdl.handle.net/123456789/24184 |
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| Language |
English
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