Molecular mapping and characterization of an RGA locus RGAPtokin1-2(171) in chickpea
Metadata of CSIR Papers
View Archive Info| Field | Value | |
| Title |
Molecular mapping and characterization of an RGA locus RGAPtokin1-2(171) in chickpea
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| Creator |
Rajesh, PN
Tekeoglu, M Gupta, VS Ranjekar, PK Muehlbauer, FJ |
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| Subject |
Agronomy; Plant Sciences; Horticulture
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| Description |
Resistance gene analog polymorphism (RGAP) is a targeted homology based method, which has been used in different crops to identify tightly linked markers for disease resistance genes and also to enrich the map with a different class of markers. In chickpea, using the RGA primers, which are designed based on the conserved motifs present in characterized R-genes, Bulk Segregant Analysis (BSA) was performed on a resistant bulk and a susceptible bulk along with parents for ascochyta blight resistance. Of all available RGAs and their 48 different combinations, only one RGA showed polymorphism during BSA. This marker was evaluated in an F-7:8 population of 142 RILs from an interspecific cross of C. arietinum (FLIP 84-92C) x C. reticulatum ( PI 599072) and was mapped to Cicer linkage map. The genomic location of chickpea RGA was compared with the locations of mapped chickpea R-genes. This is the first RGA marker mapped to chickpea linkage map.
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| Publisher |
KLUWER ACADEMIC PUBLDORDRECHTVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS
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| Date |
2011-09-24T09:57:46Z
2011-09-24T09:57:46Z 2002 |
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| Type |
Article
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| Identifier |
EUPHYTICA
0014-2336 http://hdl.handle.net/123456789/24219 |
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| Language |
English
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