Study on 16s rRNA based PCR method for specific detection of <i>Salmonella entrica typhi</i> from gut of infected silkworm <i>Bombyx mori (Linn.)</i>
IR@NISCAIR: CSIR-NISCAIR, New Delhi - ONLINE PERIODICALS REPOSITORY (NOPR)
View Archive Info| Field | Value | |
| Creator |
Pradhap, M
Selvisabhanayakam Mathivanan, V Parthasarathy, V Ayyappan, J V Ananda Kumar, S Sathees |
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| Date |
2011-10-31T10:08:17Z
2011-10-31T10:08:17Z 2011-11 |
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| Identifier |
0975-1084 (Online); 0022-4456 (Print)
http://hdl.handle.net/123456789/12959 |
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| Description |
909-911
This study presents a novel molecular method for detection of <i>Salmonella entrica typhi </i>strain based on 16s rRNA sequence analysis by modification of PCR primer 16sF1, which was combined with a newly developed primer. Annealing method at the position of 50°C PCR amplification of 16s rRNA was performed with universal primer 27F and 1429R. Combination of these two specific primers proved specific detection of <i>Salmonella entrica typhi</i>, which was isolated and cultured from gut of silk worm <i>Bombyx mori </i>(Linn.). |
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| Language |
en_US
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| Publisher |
NISCAIR-CSIR, India
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| Rights |
<img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>
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| Source |
JSIR Vol.70(11) [November 2011]
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| Subject |
PCR
Primer Primer annealing Proteobacteria <i>Salmonella</i> |
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| Title |
Study on 16s rRNA based PCR method for specific detection of <i>Salmonella entrica typhi</i> from gut of infected silkworm <i>Bombyx mori (Linn.)</i>
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| Type |
Article
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