Media engineering for production of poly-<img src='/image/spc_char/beta.gif' border=0>-hydroxybutyrate by <i>Bacillus</i> <i>firmus </i>NII 0830
IR@NISCAIR: CSIR-NISCAIR, New Delhi - ONLINE PERIODICALS REPOSITORY (NOPR)
View Archive Info| Field | Value | |
| Creator |
Deepthi, Sreelatha K
Binod, Parameswaran Sindhu, Raveendran Pandey, Ashok |
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| Date |
2011-10-31T10:22:08Z
2011-10-31T10:22:08Z 2011-11 |
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| Identifier |
0975-1084 (Online); 0022-4456 (Print)
http://hdl.handle.net/123456789/12968 |
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| Description |
968-975
This study evaluates biodiesel industry generated crude glycerol as sole carbon source for the production of poly-<img src='/image/spc_char/beta.gif' border=0>-hydroxybutyrate (PHB) using <i>Bacillus firmus </i>NII 0830. Pretreatment of crude glycerol from biodiesel industry was tried using strong acids. Biomass yield and PHB accumulation by <i>B. firmus </i>NII 0830 in submerged fermentation was compared using crude as well as pretreated glycerol as sole carbon source. It was observed that crude glycerol from biodiesel industry, without any pretreatment, can be used for PHB production. The organism when grown in mineral salt media containing crude glycerol as carbon source gave a maximum yield of biomass (3 g/l) and PHB (53% of cell dry wt). Response surface methodology (RSM) showed that presence of nitrogen and phosphate in fermentation media favored biomass production but reduced PHB production. Optimized crude glycerol media for maximum PHB accumulation contains ammonium sulphate (4 g/l) and K2HPO4 (0.8 g/l). |
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| Language |
en_US
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| Publisher |
NISCAIR-CSIR, India
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| Rights |
<img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>
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| Source |
JSIR Vol.70(11) [November 2011]
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| Subject |
Biodiesel industry
Biopolymer Glycerol Poly-<img src='/image/spc_char/beta.gif' border=0>-hydroxybutyrate (PHB) Response Surface Methodology (RSM) |
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| Title |
Media engineering for production of poly-<img src='/image/spc_char/beta.gif' border=0>-hydroxybutyrate by <i>Bacillus</i> <i>firmus </i>NII 0830
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| Type |
Article
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