Impact of infectious <i style="">Candida albicans</i> biofilm on biomaterials
IR@NISCAIR: CSIR-NISCAIR, New Delhi - ONLINE PERIODICALS REPOSITORY (NOPR)
View Archive Info| Field | Value | |
| Creator |
Singh, Nivedita
Agrawal, Vishnu Pemmaraju, Suma C Panwar, Richa Pruthi, Vikas |
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| Date |
2011-10-31T10:33:15Z
2011-10-31T10:33:15Z 2011-10 |
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| Identifier |
0975-0967 (Online); 0972-5849 (Print)
http://hdl.handle.net/123456789/12978 |
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| Description |
417-422
In the present investigation, biofilm formation by <i style="">Candida albicans</i> was studied on different polymeric surfaces, viz., polypropylene (PP), polystyrene (PS), polyvinylchloride (PVC), and silicone rubber (SR). Amongst these polymeric surfaces, the maximum biofilm formation was recorded to be 64.19, 50.31, and 45.09% for PS, PP, SR, respectively in comparison to PVC after 48 h using XTT [2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)2H-tetrazolium-carboxanilide] tetrazolium reduction assay. Exopolysaccharides (EPS) production during biofilm formation, when assessed using acetone precipitation technique, was found to be 11.45, 9.41, 8.65 and 6.95 <img src='/image/spc_char/micro.gif' border=0> g/cm<sup>2</sup> for PVC, PS, PP and SR, respectively. Atomic force microscopic and goniometric analysis showed maximum roughness (134 nm) and hydrophobicity (97°) for PVC. Confocal laser scanning microscopy (CLSM) studies revealed maximum biofilm thickness (117.5 µm) on PVC surface when analyzed by z-sectioning. Further, the data were confirmed by scanning electron microscopy (SEM) for biofilm growth on these biomaterials. It was observed that PVC as biomaterial is most susceptible for <i style="">C. albicans</i> biofilm formation, while material surface properties like roughness and hydrophobicity promotes <i style="">C. albicans</i> adhesion and biofilm development. |
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| Language |
en_US
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| Publisher |
NISCAIR-CSIR, India
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| Rights |
<img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>
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| Source |
IJBT Vol.10(4) [October 2011]
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| Subject |
Biofilm
Biomaterials <i>Candida albicans</i> CLSM XTT |
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| Title |
Impact of infectious <i style="">Candida albicans</i> biofilm on biomaterials
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| Type |
Article
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