Comparative elucidation of properties of sucrase-cellobiase co-aggregate produced in media containing sucrose by <i style="">Termitomyces clypeatus</i>
IR@NISCAIR: CSIR-NISCAIR, New Delhi - ONLINE PERIODICALS REPOSITORY (NOPR)
View Archive Info| Field | Value | |
| Creator |
Banik, Samudra Prosad
Pal, Swagata Ghorai, Shakuntala Majumder, Rajib Mukherjee, Soumya Chowdhury, Sudeshna Khowala, Suman |
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| Date |
2011-10-31T10:36:13Z
2011-10-31T10:36:13Z 2011-10 |
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| Identifier |
0975-0967 (Online); 0972-5849 (Print)
http://hdl.handle.net/123456789/12984 |
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| Description |
468-479
Production profiles and characterization of the sucrase-cellobiase (S-C) co-aggregates from the filamentous fungus <i style="">Termitomyces clypeatus</i> were compared in media containing 1% and 5% sucrose to understand the effect of cellular regulation over secretion and aggregation of these two industrially important glycosidases. The enzymes were secreted constitutively but in a high sucrose medium (5%), cellobiase secretion was reduced to a basal level of 0.03 U/mL. In intracellular, cell-bound and extracellular milieus, S/C ratios gradually declined in a predictable trend indicating participation of more cellobiase subunits for secretion. Sucrase was secreted <i style="">via</i> vacuoles in the fungus, following the same route as that of cellobiase and thus co-aggregates of S-C were present in the vacuolar fraction. The extracellular co-aggregates showed similar molecular sizes (>550 kDa) on zymography; however, SDS-PAGE revealed substantial difference in their subunit assemblies. Sucrase from the 5% medium showed a 2.6 times lower K<sub>m</sub> than 1% medium. These observations demonstrated the formation of a unique S-C co-assembly, optimally suited to its needs and accommodation of the constituent subunits, to be used for biotechnological applications. |
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| Language |
en_US
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| Publisher |
NISCAIR-CSIR, India
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| Rights |
<img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>
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| Source |
IJBT Vol.10(4) [October 2011]
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| Subject |
Constitutive secretion
Industrial glycosidase Regulated secretion Sucrase-cellobiase co-aggregate <i style="">Termitomyces clypeatus</i> |
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| Title |
Comparative elucidation of properties of sucrase-cellobiase co-aggregate produced in media containing sucrose by <i style="">Termitomyces clypeatus</i>
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| Type |
Article
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