Cloning and expression of an intronless gene encoding endoglucanase from <i style="">Aspergillus fumigatus</i> MKU1
IR@NISCAIR: CSIR-NISCAIR, New Delhi - ONLINE PERIODICALS REPOSITORY (NOPR)
View Archive Info| Field | Value | |
| Creator |
Meera, B
Vanitha, M C Ramani, G Manjula, A Gunasekaran, P |
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| Date |
2011-10-31T10:36:34Z
2011-10-31T10:36:34Z 2011-10 |
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| Identifier |
0975-0967 (Online); 0972-5849 (Print)
http://hdl.handle.net/123456789/12985 |
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| Description |
480-486
Endoglucanase (carboxymethyl cellulase) production by an alkali tolerant fungus <i style="">Aspergillus fumigatus</i> MKU1 was characterized under submerged fermentation conditions. The carboxymethyl cellulase (CMCase) activity was optimal at <i style="">p</i>H 5.0 and 60°C. Intronless gene <i style="">eng</i>61 coding for endoglucanase from <i style="">A. fumigatus </i>MKU1 was cloned and sequenced. The <i style="">eng</i>61 gene contains 993 bp ORF encoding a polypeptide of 330 amino acids. <i style="">In silico</i> analysis of the translated sequence revealed the presence of C-terminal cellulose binding module and signal peptide cleavage site between 20<sup>th</sup> and 21<sup>st</sup> amino acids. The <i style="">eng</i>61 was cloned in pET30b and expressed in <i style="">Escherichia coli </i>BL21 DE3. Thus, an intronless endoglucanase gene from an alkali tolerant strain of <i style="">A. fumigatus </i>has been cloned and expressed. |
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| Language |
en_US
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| Publisher |
NISCAIR-CSIR, India
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| Rights |
<img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>
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| Source |
IJBT Vol.10(4) [October 2011]
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| Subject |
<i style="">Aspergillus fumigatus</i>
Carboxymethyl cellulose Cellulase Cloning Endoglucanase Expression |
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| Title |
Cloning and expression of an intronless gene encoding endoglucanase from <i style="">Aspergillus fumigatus</i> MKU1
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| Type |
Article
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