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A novel halotolerant xylanase from marine isolate Bacillus subtilis cho40: gene cloning and sequencing

IR@NIO: CSIR-National Institute Of Oceanography, Goa


Field	Value

Creator	Khandeparker, R. Verma, P. Deobagkar, D.

Date	2011-11-14T10:50:43Z 2011-11-14T10:50:43Z 2011

Identifier	New Biotechnology, vol.28(6); 2011; 814-821 http://drs.nio.org/drs/handle/2264/3954

Description	A novel halotolerant xylanase from marine bacterium Bacillus subtilis cho40 isolated from Chorao island of Mandovi estuary Goa, India has been reported. Extracellular xylanase was produced by using agricultural residue such as wheat bran as carbon source under solid-state fermentation (SSF). The optimal pH and temperature of xylanase were reported to be 6.0 and 60 degrees C, respectively. Xyn40 was highly salt-tolerant, and showed highest activity at 0.5 M NaCl. Xylanase activity was greatly induced (140%) when pre-incubated with 0.5 M NaCl for 4 h. The xylanase gene, xyn40, from marine bacterium B. subtilis cho40 was cloned, and expressed in Escherichia coli. The xylanase gene was 645 bp long and had a 215 amino acid ORF protein with a molecular mass of 22.9 kDa. It had all features of xylanase enzyme and showed homology to xylanases reported from B. subtilis. It differs from the earlier reported xylanase sequences by the presence of more serine residues compared to threonine and also by the presence of polar (hydrophilic) amino acids in higher abundance (61%) than non-polar amino acids (39%). The novel xylanase, reported in this study is a halotolerant enzyme from marine isolate and can play a very important role in bioethanol production from marine seaweeds.

Language	en

Publisher	Elsevier

Rights	An edited version of this paper was published by Elsevier. Copyright [2011] Elsevier

Subject	enzymes biotechnology Bacillus subtilis seaweeds

Title	A novel halotolerant xylanase from marine isolate Bacillus subtilis cho40: gene cloning and sequencing

Type	Journal Article