Peptide-Protein Interactions Suggest That Acetylation of Lysines 381 and 382 of p53 Is Important for Positive Coactivator 4-p53 Interaction.
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Peptide-Protein Interactions Suggest That Acetylation of
Lysines 381 and 382 of p53 Is Important for Positive
Coactivator 4-p53 Interaction.
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| Creator |
Debnath, Subrata
Chatterjee, Snehajyoti Arif, Mohammed Kundu, Tapas K Roy, Siddhartha |
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| Subject |
Structural Biology & Bioinformatics
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| Description |
The human transcriptional positive coactivator 4 (PC4) activates several p53-dependent genes. It has been demonstrated that this is a consequence of direct interaction with p53. Previously,we have concluded that PC4 interacts mainly with the C-terminal negative regulatory domain of p53 through its DNA binding C-terminal half.NMRchemical shift perturbation studies with peptide fragments indicated that amino acids 380–386of p53 are crucial for interaction with PC4. This was verified by fluorescence anisotropy and sedimentation velocity studies. peptide consisting of p53-(380–386) sequence, when attached to a cell penetration tag and nuclear localization signal, localizes to the nucleus and inhibits luciferase gene expression from a transfected plasmid carrying a Luc gene under a p53-dependent promoter. Acetylation of lysine 382/381 enhanced the binding of this peptide to PC4 by about an order of magnitude.NMRand mutagenesis studies indicated that serine 73 of PC4 is an important residue for recognition of p53. Intermolecular nuclear Overhauser effect placed aspartate 76 in the vicinity of lysine381, indicating that the region around residues 73–76 of PC4 is important for p53 recognition. We conclude that the 380–386 region of p53 interacts with the region around residues 73–76 of PC4, and acetylation of lysine 382/381 of p53 may play an important role in modulating p53-PC4 interaction and as a consequence PC4 mediated activation of p53 target genes.
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| Date |
2011
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| Type |
Article
PeerReviewed |
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| Relation |
http://dx.doi.org/10.1074/jbc.M110.205328
http://www.eprints.iicb.res.in/42/ |
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| Identifier |
Debnath, Subrata and Chatterjee, Snehajyoti and Arif, Mohammed and Kundu, Tapas K and Roy, Siddhartha (2011) Peptide-Protein Interactions Suggest That Acetylation of Lysines 381 and 382 of p53 Is Important for Positive Coactivator 4-p53 Interaction. THE JOURNAL OF BIOLOGICAL CHEMISTRY, 286 (28). pp. 25076-25087.
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