Leishmania Donovani Glycosphingolipid Facilitates Antigen Presentation by inducing Relocation of CD1d into Lipid Rafts in Infected Macrophages
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Leishmania Donovani Glycosphingolipid Facilitates
Antigen Presentation by inducing Relocation of CD1d
into Lipid Rafts in Infected Macrophages
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| Creator |
Karmakar, Subir
Paul, Joydeep Tripti, De |
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| Subject |
Infectious Diseases and Immunology
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| Description |
NKT cells respond to presentation of specific glycolipids with release of both Th1- and
Th2-type cytokines. Leishmania donovani (LD)-infected splenic macrophages (sM/(I)) and
bone marrow-derived dendritic cells (BMDC(I)) failed to activate NKT cells in response to
a-galactosyl ceramide (a-GalCer). The defective antigen presentation could be corrected by
treating the cells with the immunostimulating glycosphingophospholipid (GSPL) of LD
parasites. In vitro pulsing of BMDC(I) or sM/(I) with GSPL, caused the activation of the
Va141 CD1d1-specific NKT cell hybridoma DN32.D3. Localization of MHC II and CD1d
molecules to membrane lipid rafts has been suggested to play an important role in antigen
presentation. Confocal analysis clearly demonstrated that LD infection changed the
pattern of CD1d distribution to the non-lipid raft regions and this change could be reversed
by GSPL treatment. Isoelectric focusing gel shift assay indicated that GSPL binds to CD1d.
GSPL-treated but not untreated BMDC(I) formed immune synapses with NKT cells and this
was associated with calcium mobilization. In conclusion, GSPL treatment was associated
with modification of BMDC(I)/sM/(I) lipid raft structure, which is a site for immune
regulation.
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| Publisher |
John Wiley & Sons
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| Date |
2011
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/98/1/EUROPEAN_JOURNAL_OF_IMMUNOLOGY%2C_41(5)_1376%2D1387%2C_2011_[57].pdf
Karmakar, Subir and Paul, Joydeep and Tripti, De (2011) Leishmania Donovani Glycosphingolipid Facilitates Antigen Presentation by inducing Relocation of CD1d into Lipid Rafts in Infected Macrophages. European Journal of Immunology, 41. pp. 1376-1387. ISSN 0014-2980 |
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| Relation |
http://dx.doi.org/10.1002/eji.201040981
http://www.eprints.iicb.res.in/98/ |
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