Effects of Arginine and Other Solution Additives on the Self-Association of Different Surfactants: An Investigation at Single-Molecule Resolution
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Effects of Arginine and Other Solution Additives on the
Self-Association of Different Surfactants: An Investigation
at Single-Molecule Resolution
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| Creator |
Haldar, Shubhasis
Chattopadhyay, Krishnananda |
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| Subject |
Structural Biology & Bioinformatics
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| Description |
Fluorescence correlation spectroscopy is used to
monitor the self-association of SDS and DTAB monomers at
single-molecule resolution. Tetramethylrhodamine-5-maleimide (TMR)
has been chosen as a probe because rhodamine dyes have been
shown to bind surfactant micelles. Correlation functions obtained by
FCS experiments have been fit using conventional discrete diffusional
component analysis as well as the more recent maximum
entropy method (MEM). Hydrodynamic radii calculated from the
diffusion time values increase with surfactant concentration as the
monomers self-associate. Effects of several solution additives on the
self-association property of the surfactants have been studied. Urea
and glycerol inhibit self-association, and arginine shows a dual nature.
With SDS, arginine favors self-association, and with DTAB, it inhibits
micelle formation. We propose surfactant self-association to be a
“supersimplified” model of protein aggregation.
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| Publisher |
American Chemical Society
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| Date |
2011
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/105/1/LANGMUIR%2C27(10)_5842%2D5849%2C2011[48].pdf
Haldar, Shubhasis and Chattopadhyay, Krishnananda (2011) Effects of Arginine and Other Solution Additives on the Self-Association of Different Surfactants: An Investigation at Single-Molecule Resolution. Langmuir, 27 (10). pp. 5842-5849. |
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| Relation |
http://dx.doi.org/10.1021/la200580z
http://www.eprints.iicb.res.in/105/ |
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