Four new dinuclear Cu(II) hydrazone complexes using various organic spacers: syntheses, crystal structures, DNA binding and cleavage studies and selective cell inhibitory effect towards leukemic and normal lymphocytes
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Four new dinuclear Cu(II) hydrazone complexes using various organic spacers:
syntheses, crystal structures, DNA binding and cleavage studies and selective
cell inhibitory effect towards leukemic and normal lymphocytes
|
|
| Creator |
Banerjee, Sambuddha
Mondal , Susmita Sen, Soma Das, Saurabh Hughes, David L Rizzoli, Corrado Desplanches, C´edric Mandal, Chitra Mitra, Samiran |
|
| Subject |
Infectious Diseases and Immunology
|
|
| Description |
Syntheses and crystal structures of four new hydrazone-based Cu(II) complexes,
[{Cu(L1)(H2O)}2(m-pyraz)](ClO4)2 (1), [{Cu(L1)(OClO3)}2(m-4,4¢-bipy)] (2),
[{Cu(L2H)}(m-pyraz){Cu(L2H)(OClO3)}].(ClO4) (3) and [{Cu(L2)}2(m-bpe)] (4) [L1H = condensation
product of benzhydrazide and pyridine-2-carbaldehyde and L2H2 = condensation product of benzoyl
acetone and benzhydrazide], bridged by various organic spacers [pyrazine (pyraz), 4,4¢-bipyridine
(4,4¢-bipy) and 1,2-di(4-pyridyl)ethane (bpe)] are reported in this paper. The single-crystal X-ray
crystallographic studies reveal that all are dinuclear units where 1 and 3 form strong intermolecular
H-bonding to form sheets of interconnected ions, whereas 2 forms sheets of dinuclear chains through
p–p interactions; in 4, molecules are linked only through van der Waals interactions. The
variable-temperature magnetic moment studies reveal that 1 and 3 show antiferromagnetic coupling
between the Cu(II) centers at lower temperatures. The binding ability of 1 with calf thymus DNA
[CT-DNA] is reported using various spectroscopic studies (UV-Vis titration, circular dichroism and
fluorescence). The binding constants of 1 with CT-DNA, as calculated by different methodologies, are
of the order of 105 M-1. The mode of interaction between 1 and CT-DNA has been predicted using
circular dichroic (CD) spectroscopy, where it has been shown that 1 most probably interacts with DNA
via intercalation between the base pairs leading to a change in B-DNA conformation. 1 is also able to
cleave supercoiled (SC) plasmid DNA pUC19 in a time and dose dependent manner as demonstrated
by agarose gel electrophoresis, and also demonstrates its potential to cleave the SC plasmid DNA via
both oxidative and hydrolytic mechanisms. Approximately 50% of leukemic cells are found to be dead
when two representative leukemic cell lines are exposed to 1 (~80 mM) even for 24 h as determined by
different cell cytotoxicity assays. Preliminary results also showed that, at 20 mM, 1 could selectively
induce apoptosis in leukemic cells without affecting normal lymphocytes.
|
|
| Date |
2009
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://www.eprints.iicb.res.in/146/1/DALTON_TRANSACTIONS%2C34%2C_6849%2D6860%2C2009[138].pdf
Banerjee, Sambuddha and Mondal , Susmita and Sen, Soma and Das, Saurabh and Hughes, David L and Rizzoli, Corrado and Desplanches, C´edric and Mandal, Chitra and Mitra, Samiran (2009) Four new dinuclear Cu(II) hydrazone complexes using various organic spacers: syntheses, crystal structures, DNA binding and cleavage studies and selective cell inhibitory effect towards leukemic and normal lymphocytes. Dalton Transactions. pp. 6849-6860. |
|
| Relation |
http://dx.doi.org/10.1039/b903072g
http://www.eprints.iicb.res.in/146/ |
|