Stimulation of Menthol Production in Mentha Piperita Cell Culture
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Stimulation of Menthol Production in Mentha Piperita Cell Culture
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| Creator |
Chakraborty, Amrita
Chattopadhyay, Sharmila |
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| Subject |
Drug Development/Diagnostics & Biotechnology
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| Description |
Plant cell culture provides an alternative means
for producing secondary metabolites. In this study, experiments were carried out to study the impact of several
parameters, independently and in combination, on the
stimulation of menthol production in the cell suspension
culture of Mentha piperita. Callus was obtained from leaf
segments of in vitro grown plantlets on Murashige and
Skoog (MS) medium supplemented with 0.2 mg l−1of 2,4-
dichlorophenoxy acetic acid to initiate cell suspension
culture. This culture was maintained in half-strength MS
medium supplemented with 0.2 mg l−1of 2,4-dichlorophenoxy
acetic acid at 15 d interval and used for further
studies. Precursor feeding alone, i.e., menthone, at 35 μM
concentration showed slightly improved productivity. γ-
Cyclodextrin alone at 60 μM concentration and in
combination with menthone feeding at 35 μM increased
menthol yield up to 92 and 110 mg l−1 in comparison to
77 mg l−1 of control culture. Synergistic potentiation effect
of menthone feeding at 35 μM and γ-cyclodextrin at
60 μM treatment followed by in situ adsorption with RP-
8 also showed potential stimulation of menthol production
in M. piperita cell culture. Fungal elicitor treatment showed
enhanced production level up to 140.8 mg l−1 in comparison
to that of control. Further studies were carried out with
the establishment of Agrobacterium tumefaciens (Ach5) gall-mediated calli, and consequently, cell suspension
culture and results showed the significant enhancement of
menthol yield up to 278 mg l−1.
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| Date |
2008
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/155/1/IN_VITRO_CELLULAR_%26_DEVELOPMENTAL_BIOLOGY%2DPLANT%2C_44(_6)%2C_518%2D524%2C_2008[7].pdf
Chakraborty, Amrita and Chattopadhyay, Sharmila (2008) Stimulation of Menthol Production in Mentha Piperita Cell Culture. In Vitro Cell.Dev.Biol.-Plant, 44. pp. 518-524. |
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| Relation |
http://dx.doi.org/10.1007/s11627-008-9145-y
http://www.eprints.iicb.res.in/155/ |
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