RNA-Binding Potential of Protoberberine Alkaloids: Spectroscopic and Calorimetric Studies on the Binding of Berberine, Palmatine, and Coralyne to Protonated RNA Structures
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
RNA-Binding Potential of Protoberberine Alkaloids:
Spectroscopic and Calorimetric Studies
on the Binding of Berberine, Palmatine,
and Coralyne to Protonated RNA Structures
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| Creator |
Islam, Md. Maidul
Kumar, G Suresh |
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| Subject |
Chemistry
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| Description |
Interaction of the protoberberine alkaloids berberine, palmatine, and coralyne with the two double-stranded
RNA homopolymers of cytidine–guanosine (CG) and inosine–cytidine (IC) sequences in the protonated
conformation was investigated using various biophysical techniques. All the three alkaloids bound polyCþG in
a cooperative way. The binding of coralyne to both the polyribonucleotides was stronger than that of berberine
and palmatine. Evidence for the intercalative binding of coralyne was revealed from fluorescence quenching
studies. Isothermal titration calorimetry results suggested that the binding of berberine to both
the polymers and palmatine to polyICþ was very weak while that of palmatine and coralyne to polyCþG and
polyICþ was predominantly entropy driven. Circular dichroic results provided evidence for the perturbation
of the RNA conformation with the bound coralyne in a more deeply intercalated position compared to berberine
and palmatine as revealed by induced circular dichroism peaks. Taken together, the present study
suggests that planarity of coralyne results in a more favorable and stronger binding to the double-stranded
RNA conformations compared to berberine and palmatine that may potentiate its use in RNA-targeted drug
design.
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| Publisher |
Mary Ann Liebert
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| Date |
2009
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/156/1/DNA_AND_CELL_BIOLOGY%2C28(12)%2C637%2D650%2C2009[5].pdf
Islam, Md. Maidul and Kumar, G Suresh (2009) RNA-Binding Potential of Protoberberine Alkaloids: Spectroscopic and Calorimetric Studies on the Binding of Berberine, Palmatine, and Coralyne to Protonated RNA Structures. DNA and Cell Biology, 28 (12). pp. 637-650. |
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| Relation |
http://www.eprints.iicb.res.in/156/
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