Laccase production in solid-state and submerged fermentation by Pleurotus ostreatus
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Laccase production in solid-state and
submerged fermentation by Pleurotus
ostreatus
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| Creator |
Mazumder, Sharmistha
Basu, Soumendra K Mukherjee, Mina |
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| Subject |
Drug Development/Diagnostics & Biotechnology
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| Description |
A solid-state fermentation (SSF) system for production of an industrially
important enzyme laccase by Pleurotus ostreatus was developed by using potato
dextrose yeast extract medium and polyurethane foam as a supporting material.
The maximum laccase production in the SSF system was as high as 3�105 U/L.
Addition of inducers, such as copper and ferulic acid, further enhanced the laccase
production in SSF. Moreover, the time required for the maximum laccase production
was reduced to 6 days compared to 10 days reported earlier. The improvement
achieved by the SSF system was investigated by comparing it to a
submerged fermentation system (SmF), both experimentally and by using a
standard theoretical model along with a parameter sensitivity analysis. Laccase
production in SSF was found to be twice of that in SmF. One of the main reasons
for higher laccase production in SSF compared to SmF was possibly due to the
presence of higher proteolytic activity in SmF. Strong proteolytic activity in SmF
presumably caused subsequent laccase degradation, which lowered the ultimate
laccase production in SmF compared to SSF.
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| Publisher |
John Wiley & Sons
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| Date |
2009
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/157/1/ENGINEERING_IN_LIFE_SCIENCES%2C_99(1)%2C45%2D52%2C2009[105].pdf
Mazumder, Sharmistha and Basu, Soumendra K and Mukherjee, Mina (2009) Laccase production in solid-state and submerged fermentation by Pleurotus ostreatus. Engineering in Life Science, 9 (1). pp. 45-52. ISSN 1618-0240 |
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| Relation |
http://dx.doi.org/10.1002/elsc.200700039
http://www.eprints.iicb.res.in/157/ |
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