Complete protection against experimental visceral leishmaniasis with complete soluble antigen from attenuated Leishmania donovani promastigotes involves Th1-immunity and down-regulation of IL-10
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Complete protection against experimental visceral
leishmaniasis with complete soluble antigen from
attenuated Leishmania donovani promastigotes involves
Th1-immunity and down-regulation of IL-10
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| Creator |
Bhaumik , Siddhartha Kumar
Naskar, Kshudiram De, Tripti |
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| Subject |
Infectious Diseases and Immunology
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| Description |
Compared with cutaneous leishmaniasis, vaccination against visceral leishmaniasis has
received limited attention. Most available drugs are toxic, and relapse after cure remains a
chronic problem. Growing limitations in available chemotherapeutic strategies due to emerging
resistant strains and lack of an effective vaccine strategy against visceral leishmaniasis deepens
the crisis. Complete soluble antigen (CSA), from a b1-4 galactosyltransferase expressing attenuated
Leishmania donovani parasite, induced protection against subsequent challenge and
during active infections. CSA immunization was effective against both pentavalent antimony
sensitive and resistant strains of L. donovani. Majority (�85%) of the immunized animals
showed sterile protection. Resolution of the disease required the presence of T cells, and the
recovered animals remained immune to re-challenge. Control of the parasites was dependent
on type 1 CD41 helper cells, which evolved in the presence of IL-12 and activatedmacrophages
through the production of IFN-c. Immunity was adoptively transferable and was dependent on
both CD41 and CD81 cells. CSA immunization led to enhanced IFN-c production, while
suppressing the IL-10 production. However, CSA immunization did not abrogate IL-4 production.
Our results accentuate the need to establish a favorable cellular immunity while intervening
with the development of Th2 cells during leishmania infection.
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| Publisher |
John Wiley & Sons
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| Date |
2009
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/160/1/EUROPEAN_JOURNAL_OF_IMMUNOLOGY%2C39(_8)_%2C2146%2D2160%2C_2009[39].pdf
Bhaumik , Siddhartha Kumar and Naskar, Kshudiram and De, Tripti (2009) Complete protection against experimental visceral leishmaniasis with complete soluble antigen from attenuated Leishmania donovani promastigotes involves Th1-immunity and down-regulation of IL-10. European Journal of Immunology, 39 (8). pp. 2146-2160. ISSN 0014-2980 |
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| Relation |
http://dx.doi.org/10.1002/eji.200839017
http://www.eprints.iicb.res.in/160/ |
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