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In vitro and in vivo activity of Aloe vera leaf exudate in experimental visceral leishmaniasis

IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata


Field	Value

Title	In vitro and in vivo activity of Aloe vera leaf exudate in experimental visceral leishmaniasis

Creator	Dutta, Avijit Sarkar, Debjani Gurib-Fakim, Ameenah Mandal, Chitra Chatterjee, Mitali

Subject	Infectious Diseases and Immunology

Description	The leishmanicidal activity of Aloe vera leaf exudate (AVL) has been demonstrated in promastigotes and axenic amastigotes, but its effectiveness in animal models has not been evaluated. The presence of alkaloids, triterpenes, cyanidines, proanthocyanidines, tannins, and saponins in AVL was identified. Its effectiveness in four Leishmania donovani strains was studied both in promastigotes (IC50 ranged from 70–115 μg/ml) and amastigotes (IC50 ranged from 3.1–11.4 μg/ml). In amastigotes, the killing by AVL was facilitated through its induction of nitric oxide in leishmania-infected macrophages. The safety index was good as AVL up to 300 μg/ml remained nontoxic to monocytes and macrophages. In a L. donovani BALB/c mouse model, oral or subcutaneous administration of AVL (15 mg/kg body weight×5 days) reduced parasitemia by >90% in the liver, spleen, and bone marrow without impairment of hepatic and renal functions. Collectively, we conclude that AVL shows promising antileishmanial activity and may provide a new lead agent in the treatment of Leishmaniasis.

Date	2008

Type	Article PeerReviewed

Format	application/pdf

Identifier	http://www.eprints.iicb.res.in/188/1/PARASITOLOGY_RESEARCH%2C102(_6)%2C1235%2D1242%2C2008[94].pdf Dutta, Avijit and Sarkar, Debjani and Gurib-Fakim, Ameenah and Mandal, Chitra and Chatterjee, Mitali (2008) In vitro and in vivo activity of Aloe vera leaf exudate in experimental visceral leishmaniasis. Parasitol Res, 102 (6). pp. 1235-1242.

Relation	http://dx.doi.org/10.1007/s00436-008-0899-2 http://www.eprints.iicb.res.in/188/