Reactive Oxygen Species and Imbalance of Calcium Homeostasis Contributes to Curcumin Induced Programmed Cell Death in Leishmania donovani
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Reactive Oxygen Species and Imbalance of Calcium Homeostasis
Contributes to Curcumin Induced Programmed Cell Death
in Leishmania donovani
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| Creator |
Das, Rakhee
Roy, Amit Dutta, Neeta Majumder, Hemanta K |
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| Subject |
Infectious Diseases and Immunology
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| Description |
Curcumin, a polyphenol compound, has been
recognized as a promising anti-cancer drug. The purpose of
the present study was to investigate the cytotoxicity of
curcumin to Leishmania donovani, the causative agent for
visceral leishmaniasis. Flow cytometric analysis revealed
that curcumin induced cell cycle arrest at G2/M phase.
Incubation of Leishmania promastigotes with curcumin
caused exposure of phosphatidylserine to the outer leaflet
of plasma membrane. This event is preceded by curcumininduced
formation of reactive oxygen species (ROS) and
elevation of cytosolic calcium through the release of calcium
ions from intracellular stores as well as by influx of
extracellular calcium. Elevation of cytosolic calcium is
responsible for depolarization of mitochondrial membrane
potential (DWm), release of Cytochrome c into the cytosol
and concomitant nuclear alterations that included
deoxynucleotidyltransferase-mediated dUTP end labeling
(TUNEL) and DNA fragmentation. Taken together, these
data indicate that curcumin has promising antileishmanial
activity that is mediated by programmed cell death and,
accordingly, merits further investigation as a therapeutic
option for the treatment of leishmaniasis.
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| Date |
2008
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/286/1/APOPTOSIS%2C13(7)%2C867%2D882%2C2008[63].pdf
Das, Rakhee and Roy, Amit and Dutta, Neeta and Majumder, Hemanta K (2008) Reactive Oxygen Species and Imbalance of Calcium Homeostasis Contributes to Curcumin Induced Programmed Cell Death in Leishmania donovani. Apoptosis, 13 (7). pp. 867-882. |
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| Relation |
http://dx.doi.org/10.1007/s10495-008-0224-7
http://www.eprints.iicb.res.in/286/ |
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