Characterization of a dimeric unfolding intermediate of bovine serum albumin under mildly acidic condition
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Characterization of a dimeric unfolding intermediate of bovine serum albumin under mildly acidic condition
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| Creator |
Brahma, Amrita
Mandal, Chhabinath Bhattacharyya, Debasish |
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| Subject |
Drug Development/Diagnostics & Biotechnology
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| Description |
Protein aggregation is a well-known phenomenon related to serious medical implications. Bovine serum albumin (BSA), a structural analogue of human serum albumin, has a natural tendency for aggregation under stress conditions. While following effect of moderately acidic pH on BSA, a state was identified at pH 4.2 having increased light scattering capability at 350 nm. It was essentially a dimer devoid of
disulphide linked large aggregates as observed from Fspin column_ experiments, gel electrophoresis and ultra-centrifugations. Its surface hydrophobic character was comparable to the native conformer at pH 7.0 as observed by the extraneous fluorescence probes pyrene and
pyrene maleimide but its interactions with 1-anilino 8-naphthelene sulphonic acid was more favorable. Dimerization was irreversible between pH 4.2 and 7.0 even after treatment with DTT. The role of the only cysteine-34 residue was investigated where modification with reagents of
arm length bigger than 6 A° prevented dimerization. Molecular modeling of BSA indicated that cys-34 resides in a cleft of 6 A° depth. This indicated that the area surrounding the cleft plays important role in inducing the dimerization.
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| Date |
2005
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
application/pdf |
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| Identifier |
http://www.eprints.iicb.res.in/334/1/BIOCHIMICA_ET_BIOPHYSICA_ACTA%2DPROTEINS_AND_PROTEOMICS_1751(_2)159%2D169_;2005[38].pdf
http://www.eprints.iicb.res.in/334/2/BIOCHIMICA_ET_BIOPHYSICA_ACTA%2DPROTEINS_AND_PROTEOMICS_1751(_2)159%2D169_;2005[38].pdf Brahma, Amrita and Mandal, Chhabinath and Bhattacharyya, Debasish (2005) Characterization of a dimeric unfolding intermediate of bovine serum albumin under mildly acidic condition. BBA - Biochimica et Biophysica Acta, 1751. 159 -169. |
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| Relation |
http://dx.doi.org/10.1016/j.bbapap.2005.06.007
http://www.eprints.iicb.res.in/334/ |
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