Leishmania donovani Affects Antigen Presentation of Macrophage by Disrupting Lipid Rafts
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Leishmania donovani Affects Antigen Presentation of
Macrophage by Disrupting Lipid Rafts
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| Creator |
Chakraborty, Debabani
Banerjee, Subha Sen, Abhik Banerjee, Kalyan K Das, Pradip Roy, Syamal |
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| Subject |
Infectious Diseases and Immunology
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| Description |
Leishmania donovani-infected splenic macrophages and P388D1 (P388D1(I)) failed to activate T cells in response to low dose of
exogenous peptide. The membrane fluidity of P388D1(I) was greater than that of the normal counterpart P388D1(N), but could
be reduced either by exposing the cell below phase transition point or by loading cholesterol into membrane (L-P388D1(I)), and
this was associated with enhanced Ag-presenting ability of P388D1(I). Presentation of endogenous leishmanial Ag, kinetoplastid
membrane protein-11, was also defective, but could be corrected by loading cholesterol into membrane. Because membrane rafts
are important for Ag presentation at a low peptide dose, raft architecture of P388D1(I) was studied using raft (CD48 and cholera
toxin-B) and non-raft (CD71) markers in terms of their colocalization with I-Ad. Binding of anti-CD48 mAb and cholera toxin B
subunit decreased significantly in P388D1(I), and consequently, colocalization with I-Ad was not seen, but this could be restored
in L-P388D1(I). Conversely, colocalization between I-Ad and CD71 remained unaffected regardless of the presence or the absence
of intracellular parasites. P388D1(N) and L-P388D1(I), but not P388D1(I), formed peptide-dependent synapse with T cells quite
efficiently and this was found to be corroborated with both intracellular Ca2� mobilization in T cells and IL-2 production. This
indicated that intracellular parasites disrupt the membrane rafts, possibly by increasing the membrane fluidity, which could be
corrected by making the membrane rigid. This may be a strategy that intracellular L. donovani adopts to evade host immune
system. The Journal of Immunology, 2005, 175: 3214–3224.
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| Publisher |
American Association of Immunologists
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| Date |
2005
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/336/1/BIOORGANIC_%26_MEDICINAL_CHEMISTRY_13(16)4851%2D4863_;2005[37].pdf
Chakraborty, Debabani and Banerjee, Subha and Sen, Abhik and Banerjee, Kalyan K and Das, Pradip and Roy, Syamal (2005) Leishmania donovani Affects Antigen Presentation of Macrophage by Disrupting Lipid Rafts. The Journal of Immunology, 175. pp. 3214-3224. ISSN 0022-1767 |
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| Relation |
http://dx.doi.org/
http://www.eprints.iicb.res.in/336/ |
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