Regulation and Structural Organization of Multimeric Proteins
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Regulation and Structural Organization of Multimeric Proteins
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| Creator |
Nupur, Banerjee
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| Subject |
Structural Biology & Bioinformatics
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| Description |
purified proteins, by which, the mechanism of a protein’s activity and regulation
can be studied under controlled environments. Protein purification, therefore, is
the primary prerequisite for in vitro studies. Different approaches are being
adopted to purify a protein up to homogeneity, starting from cell lysis, by which
the whole cellular content is released into a solution forming ‘cell lysate’. This is
then purified by different degrees of ultracentrifugation, precipitation of proteins
by ‘salting out’, various types of chromatographic, electrophoretic and
spectroscopic techniques involving different properties of the protein, etc.
Nowadays, the most popular and simplest way to obtain large amount of
sufficiently pure protein is genetic engineering, e. g., the ‘his-tag’ method.
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| Date |
2010
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| Type |
Thesis
NonPeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/339/1/Thesis%2DFinal.pdf
Nupur, Banerjee (2010) Regulation and Structural Organization of Multimeric Proteins. PhD thesis, Jadavpur University. |
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| Relation |
http://www.eprints.iicb.res.in/339/
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