Solvation Dynamics of a Protein in the Pre Molten Globule State
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Solvation Dynamics of a Protein in the Pre Molten Globule State
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| Creator |
Samaddar, Soma
Mandal, Amit Kumar Mondal, Sudip Kumar Sahu, Kalyanasis Bhattacharyya, Kankan Roy, Siddhartha |
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| Subject |
Structural Biology & Bioinformatics
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| Description |
The nature of solvent molecules around proteins in native and different non-native states is crucial for
understanding the protein folding problem. We have characterized two compact denatured states of glutaminyltRNA
synthetase (GlnRS) under equilibrium conditions in the presence of a naturally occurring osmolyte,
L-glutamate. The solvation dynamics of the compact denatured states and the fully unfolded state has been
studied using a covalently attached probe, acrylodan, near the active site. The solvation dynamics progressively
becomes faster as the protein goes from the native to the molten globule to the pre molten globule to the fully
unfolded state. Anisotropy decay measurements suggest that the pre-molten-globule intermediate is more
flexible than the molten globule although the secondary structure is largely similar. Dynamic light scattering
studies reveal that both the compact denatured states are aggregated under the measurement conditions. The
implications of solvation dynamics in aggregated compact denatured states have been discussed.
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| Publisher |
American Chemical Society
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| Date |
2005
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/409/1/JOURNAL_OF_PHYSICAL_CHEMISTRY_B%2C_110(42)%2C_21210%2D21215%2C[23].pdf
Samaddar, Soma and Mandal, Amit Kumar and Mondal, Sudip Kumar and Sahu, Kalyanasis and Bhattacharyya, Kankan and Roy, Siddhartha (2005) Solvation Dynamics of a Protein in the Pre Molten Globule State. The Journal of Physical Chemistry B, 110 (42). pp. 21210-21215. |
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| Relation |
http://dx.doi.org/10.1021/jp064136g CCC: $33.50
http://www.eprints.iicb.res.in/409/ |
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