Production and purification of a bioactive substance inhibiting multiple drug resistant bacteria and human leukemia cells from a salt-tolerant marine Actinobacterium sp. isolated from the Bay of Bengal
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Production and purification of a bioactive substance
inhibiting multiple drug resistant bacteria and human
leukemia cells from a salt-tolerant marine Actinobacterium
sp. isolated from the Bay of Bengal
|
|
| Creator |
Sah, Malay
Jaisankar , P Das, Satadal Sarkar, Kalyan K Roy, Soma Besra, Shila E Vedasiromani, J R Ghosh, Debashish Sana, Barindra Mukherjee, Joydeep |
|
| Subject |
Drug Development/Diagnostics & Biotechnology
|
|
| Description |
Four marine actinobacteria tolerant to
200 g NaCl l)1 were screened for antibacterial
activity against eight patient-derived multiple
drug resistant (MDR) bacteria. The active compound
(MW 300.2, predicted molecular formula
C20H28O2) from an actinobacterium, was inhibitory
to three Gram-positive and three Gramnegative
MDR bacteria, seven non-clinical
Gram-positive, four Gram-negative bacteria and
five fungi (MIC: 3.5–4.0 lg ml)1). Also, 54% of
human leukemia (HL-60) cells were killed by the
compound at 0.05 lg ml)1. Bioreactor production
demonstrated unusual primary metabolite kinetics.
Molecular phylogenetic analysis showed this
typical intertidal inhabitant to be a member of
the Streptomyces genus and distinct from other
salt-tolerant actinobacteria. As no compound was found to match the properties in several electronic
databases, our screening strategy should
increase the possibility of discovering bioactive
molecules from rare actinobacteria.
|
|
| Date |
2006
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://www.eprints.iicb.res.in/428/1/BIOTECHNOLOGY_LETTERS%2C__28(_14)%2C__1083%2D1088_[67].pdf
Sah, Malay and Jaisankar , P and Das, Satadal and Sarkar, Kalyan K and Roy, Soma and Besra, Shila E and Vedasiromani, J R and Ghosh, Debashish and Sana, Barindra and Mukherjee, Joydeep (2006) Production and purification of a bioactive substance inhibiting multiple drug resistant bacteria and human leukemia cells from a salt-tolerant marine Actinobacterium sp. isolated from the Bay of Bengal. Biotechnol Lett, 28 (14). pp. 1083-1088. |
|
| Relation |
htpp://dx.doi.org/10.1007/s10529-006-9054-2
http://www.eprints.iicb.res.in/428/ |
|